Histological, Morpho-Physiological, and Biochemical Changes during Adventitious Rooting Induced by Exogenous Auxin in Magnolia wufengensis Cuttings

Magnolia wufengensis, a rare ornamental tree species, is now in huge gap between market demand and actual supply of seedlings. As cutting propagation one the most important means to solve shortage seedling supply, this study developed an efficient procedure M. revealed morphological histological changes adventitious root formation, explored rhythm correlation rooting process physiological biochemical changes. Cuttings pre-treated with NAA:IBA (2:1) exhibited best performance. Anatomical analysis demonstrated that primordia wufengensis were initiated from cambial parenchyma cells xylem, no relationship callus formed on epidermis. The wufengenis can be divided into four periods: induction phase (0–8 dap) (dap days after planting), initiation (8–13 dap), expression (13–18 extension (18–28 dap). induced accumulations 3-indoleacetic-acid increased contents peroxidase polyphenol-oxidase near wounding at phase. phase, first modifications formation meristemoids, correspond increase peroxidase, polyphenol-oxidase, soluble protein contents. synergistic reaction low high levels gibberellins zeatin also stimulates In activities IAA-oxidase, co-stimulate emergence outgrowth roots. present not only provides optimized protocol by application auxin combination but presents insights histological, morpho-physiological, stem cuttings wufengensis.